Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42
pMA-@CC2FosMB-05000bp
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K260007_sequence (Version 1) |
Description
This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 05000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.The pMA-@CC2FosMB-05000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260007|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 05000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 5,000 bp exactly.
These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:
[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp
Notes
The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260007|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].Source
Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].| Sequence Annotation | Location | Component / Role(s) |
| suffix BBa_K260001 (pCC2FosMB) 3' homology FRT-05000bp PvuII site ColE1 origin PvuII site AmpR BBa_K260001 (pCC2FosMB) 5' homology FRT-05000bp prefix | 1,21 22,91 92,97 1617,2284 2468,2473 609,1469 2474,2543 2544,2565 | engineered_region feature/BioBrick feature/misc sequence_feature feature/dna sequence_feature sequence_feature feature/misc feature/dna sequence_feature feature/cds CDS feature/misc sequence_feature engineered_region feature/BioBrick |