BBa_K264004

BBa_K264004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K264004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adam Bower
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:42

Modular UP-element Design 5



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K264004_sequence (Version 1)

Description

The UP element is a component of the promoter located upstream of the -35 region. Though not necessary for transcription, the UP element increases the rate of transcription initiation by interacting with the alpha-subunit of RNA polymerase. A consensus sequence, -59 nnAAA(A/T)(A/T)T(A/T)TTTTnnAAAAnnn -38, was derived from a collection of many UP elements based on nucleotide frequency at each position (Estrem et al., 1998). UP Element Design 1 is the UP element with the highest transcription rate increase reported in that study. Design 1 was systematically modified (BBa_K264001-K264007) to vary the relative strength of its effect based on the hypothesis that the most significant promoter enhancement would occur when the UP element sequence used the most frequently-occurring nucleotides at each position (i.e., the consensus sequence). A spacer, CT, is placed between the UP element and subsequent promoters to maintain proper alignment with RNAP.

Notes

The observed UP elements were A-T rich. Therefore, the rational sequence modifications of Design 1 were made to preserve this feature. Nucleotides in Design 1 at positions -59, -54, and -45 were changed from G to A, A to T, and C to G, respectively. A spacer, CT, is placed between the UP element and subsequent promoters to maintain proper alignment with RNAP.

Source

Rational redesign of BBa_K264000

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K264004/1