Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:43
pSB6A0
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K268000_sequence (Version 1) |
Description
Biobricked plasmid backbone pRS314 with XbaI, PstI and SpeI sites removed from outside the MCS. One of a series of pBluescript based yeast centrometer vectors developed by Sikorski R.S., Hieter P (Genetics 122:19-27(1989)). Confers Trp resistence in yeast and ampicillan resistance in E. coli where it can be transformed and amplified for cloning purposes.Notes
Removal of non-biobrick compatible restriction sites.Source
Unmodified version can be found by accession number U03440.| Sequence Annotation | Location | Component / Role(s) |
| TRP1 F1_Ori, M13_Ori M13F AmpR Biobrick suffix ColE1_Origin Biobrick prefix CEN6_ARS4 | 817,1490 264,719 1837,1854 2870,3529 1,21 3627,4609 4793,4814 2022,2540 | CDS feature/cds sequence_feature feature/misc feature/primer_binding primer_binding_site CDS feature/cds sequence_feature feature/dna feature/misc sequence_feature feature/dna sequence_feature sequence_feature feature/dna |