Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:43
YGR035C->GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K268016_sequence (Version 1) |
Description
~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YGR035C gene. For transformation in S. cerevisiae only, this part will swap out the YGR035 gene and replace it with a Kan marker+GFP coding region downstream of the YGR035C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers:
YGR035Cdel-F: 63-mer CGGAAGTTATAAGTTATCTTCTCTACAGAAACAATACGTAATGcggatccccgggttaattaa
YGR035Cdel-R: 62-mer GAAGATGCAAAGAAGTAGTTAAGCACATTGACCTCAGGGTTGgaattcgagctcgtttaaac
Notes
Primers included ATG siteSource
Promoter region from gene YGR035C in S. cerevisiae as obtained through yeastgenome.org.Gene deletion and tagging cassette from Longtine, et al (1998).
| Sequence Annotation | Location | Component / Role(s) |
| TADH1 GFPMut3 KanR2 PTEF YGR035C_Promoter, forward primer region TTEF_Terminator YGR035C gene, reverse primer region | 799,978 74,769 1405,2214 1061,1402 1,40 2223,2435 2472,2509 | feature/stop stop_codon CDS feature/cds CDS feature/cds feature/promoter promoter promoter feature/promoter stop_codon feature/stop feature/primer_binding primer_binding_site |