Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanseong Roh
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:44
Aryl acylamidase
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K271000_sequence (Version 1) |
Description
Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-purple color, aryl acylamidase gene will apply to reporter gene.Notes
ATGGGTAAGTCACATTCGCCAGTTCACTGGAAGTCGGCGGCCGAGATCGTCGAGTTAGTCAAGAGCAAACAAATTTCCCCACGCGAGGTCGTGGAAAGCACGATCGACCTCATCGAGCAACGCGATCCCGGCCTGAATGCGGTCGTGTACAAGGCTTACG
ATGAAGCGCGCGAAAAAGCGGCTGCGCTTGAGCGGCGCATCATGCAAGGCGAGCCTGTTGGCATGCTGGCGGGGGTTCCG
ACTCTCATGAAGGACTTGTTCGCGGCCAAGCCGGGCTGGCCGTCCACGCTGGGCGGAATTCGCGCCCTCAAGGACGCCCG
CGGCGCTGCCGGCGTCTGGTCCACTTATCCATTGAAGATGTCCGGCGAAGACAGCCTGTTGCTGGGACAGACAAACAGCC
CCGTCTATGGCTTTCGCGGAACCACGGACAACACGTTTTTCGGTCCGACGCGCAATCCATTCAACCTCGACTTCAACGCA
GGGGGGTCTTCGGGAGGAGCCGCTGCGCTCGTGGCCGATGGCATCGTTCCTGTGGCAGGCGGCACGGATGGCGGCGGCTC
GATCCGCATTCCGGCGGCATGGACCAATACCTACGGCTTTCAGCCTTCGATAGGACGCGTTCCGTTCAAAAGCCCGCCCC
AATGCTTTCCATCCGGGGACCGTATCTCTATGAAGGAGCCCATCACGCGGACGGTGCCGGATGCGGCATTGGCAATGAAT
GTACTGCACGGCTTCGACCGCCGCGATCCTGCTTCGCTGCGAGTCAAGCTGGACTTCACCTCAGCGCTTGCACAAGGCGT
GCGTGGCAAAAAAATCGGCCTGACGCTGAATTACGGGGTATTTCCCGTCCAGCAAGAGATTCAGGATTTGATCGGCAAGG
CTGCCCGGGTTTTTACAGAGCTTGGCGCACACGTCGAATTCGTCGACCTCGGTATCCCCTACAGCCAGAAACAAATGAGC
GATGCCTGGTGCCGCATGATCGCGATTCCTACTGTCGCCTCGATGCAGGCGCTGCGCAAGGAAGGCATTGACTTATATGG
CGAACATCGGGCAGACATACCCGATGCGCTGATGAAGTGGATAGATGCGGTCGCTGACATAAGCGTCCAGCAGATCAGCG
CTGATCAACTATTGAGAACGACCGTTTTCGATTGCATGAACGGCGTATTCGATCGCTTTGACCTATTATTGGCGCCGACC
CTGGCATGCATGCCTGTTCGCAATGCGACTGATGGCTGCACCGAGGGACCAAGCCAGATCAACGGTGAAGAAATCGACCC
TTTGATCGGCTGGTGCATGACTTACCTGACCAATTTTTCAGGGCATCCCAGTGCCAGTGTCCCGGCTGGCCTGATTGATG
GCCTGCCGGCAGGCATGCTCATTATCGGCGATCGCCAGGCGGACCTGGATGTCATTGCCGCAAGCGCCGCTTTCGAGCGA
GCCAGCCCGTGGTCCCAATACTATGACATTCCCGCCGGACGGCCCCTGTGATAA
Source
soil bacterium| Sequence Annotation | Location | Component / Role(s) |
| PyodA Aryl acylamidase double stop codon AAV | 1,271 272,1759 1793,1798 1760,1792 | feature/promoter promoter feature/protein CDS stop_codon feature/stop feature/tag tag |