Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanseong Roh
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:44
Heavy-metal detector
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K271001_sequence (Version 1) |
Description
We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II)is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to thepromotersofmercury resistance(mer) operons, including a long spacer region containing
an inverted repeat sequence.
Notes
aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtga
tacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaac
attgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttg
taacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctgctgct
gtttaataaACTAGAcgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggt
taatATGgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaagg
tgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcTgcagcaaacgacgaaaactacgctgctgctgttTAATAA
Source
Escherichia coli K12| Sequence Annotation | Location | Component / Role(s) |
| promoter arsenate BBa_E0044 BBa_E1010 promoter zntA AAV double stop codon | 1,50 51,806 885,1559 816,884 1560,1592 1593,1598 | feature/promoter promoter CDS feature/protein CDS feature/protein feature/promoter promoter tag feature/tag feature/stop stop_codon |