BBa_K277024

BBa_K277024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K277024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:46

3L.3_23.A2.12



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K277024_sequence (Version 1)

Description

3L.3_23.A2.12 is 742 bases long and is cloned into the pGem-T vector.

3L.3_23.A2.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.12 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.13, and 3L.3_23.A2.14.)

This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
loxP_site loxPsym_YCL057W (90..123)
gene YCL056C (139..573) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
gene YCL057W (-2052..86) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins

Sequence (the first 742 bases correspond to coordinates 16956..17697 in synthetic chromosome yeast_chr3_3_23)

Notes

Constructed using overlap extension PCR protocol described in RFC38

Source

Synthetic Yeast

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K277024/1