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BBa_K283002

BBa_K283002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K283002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shi Lei, Nelson Chu, W Ling

Date created: 2009-10-12 11:00:00

Date modified: 2015-05-08 01:11:47

Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

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Details

Types	DnaRegion
Roles	Device engineered_region
Sequences	BBa_K283002_sequence (Version 1)

Description

This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.

Notes

BBa_K283002

Source

The rbs, terminator and the pLac promoter are from the registry,
the rest of the part are self designed

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0010 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_K283008 BBa_B0010 BBa_B0012	LacI BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 BBa_K283008 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_K283008 BBa_B0010 BBa_B0012	1,200 209,220 227,911 920,999 1008,1048 1057,1110 1119,1130 1137,1849 1858,1937 1946,1986	LacI BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 BBa_K283008 BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K283002/1