Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marcelo Colika Bassalo, Gabriel Francisco Zaniboni, Victor Augusti Negri and Bruno Vaz de oliveira
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:48
FinO under constitutive promoter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K284071_sequence (Version 1) |
Description
the basic protein FinO is part of the the two component FinOP system which is responsible for repressing bacterial conjugation; the FinOP system represses the transfer (tra) operon of the F-plasmid which encodes the proteins responsible for conjugative transfer of this plasmid from host to recipient Escherichia coli cells; antisense RNA, FinP is thought to interact with traJ mRNA to occlude its ribosome binding site, blocking traJ translation and thereby inhibiting transcription of the tra operon; FinO protects FinP against degradation by binding to FinP and sterically blocking the cellular endonuclease RNase E; FinO also also binds to the complementary stem-loop structures in traJ mRNA and promotes duplex formation between FinP and traJ RNA in vitro; this domain contains two independent RNA binding regions. In this device, finO is under control of pTet.Notes
no considerationsSource
Assemby of other biobricks.| Access | Instance | Definition |
| public public public public public | BBa_R0040 BBa_B0030 BBa_K284032 BBa_B0010 BBa_B0012 | p(tetR) BBa_B0030 BBa_K284032 BBa_B0010 BBa_B0012 |