BBa_K289001

BBa_K289001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K289001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nan Hou

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:49

pp1(I type Protein phosphotransferase)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K289001_sequence (Version 1)

Description

pp1(I type Protein phosphotransferase)is crucial in the MCs detection process.
According to the Toxicity mechanism, Adda binding site of MCs has a strong affinity to the lyophobic groove of PP1. Also, according to the Detoxicity mechanism, GSH could construct a covalent bond with Mdha banding site of MCs easily under the function of GST(Glutathione S-Transferase Agents which presents in the cell of Yeast).
In our project, we connect PP1 gene after DNA-BD in the plasmid pGBKT7 and GSH gene after AD in the plasmid pGADT7, and then we transform these two plasmids into Yeast(AH109) so that it can express protein DNA-BD-PP1 and AD-GSH.

Notes

it comes from homosapiam.

Source

we synthesised it in a company and did some optimization.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
misc	1,984	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P61292
• rdf-schema#seeAlso: P62140
• rdf-schema#seeAlso: P62141
• rdf-schema#seeAlso: Q5R740
• rdf-schema#seeAlso: Q8MJ47
• rdf-schema#seeAlso: P62142
• rdf-schema#seeAlso: P62143
• rdf-schema#seeAlso: P62207
• rdf-schema#seeAlso: Q3SWW9
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K289001/1

Member of these Collections

• iGEM Parts Registry