Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachel May Boyd, Phil Hall
Date created: 2010-08-11 11:00:00
Date modified: 2015-05-08 01:11:52
IPTG-inducible filamentous cell formation
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K302012_sequence (Version 1) |
Description
Bacillus subtilis in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. dinR is homologous to lexA in E. coli and is transcribed in the opposite direction of yneA.yneA stops the formation of ftsZ ring indirectly. When ftsZ forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing yneA and inhibiting ftsZ ring formation, the cells will grow filamentous.
By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ftsZ ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of yneA leads to filamentation.
Notes
noneSource
Genomic DNA from Bacillus subtilis -yneAHyperspankoid from oid and hyperspank
| Access | Instance | Definition |
| public public | BBa_K302003 BBa_K302005 | BBa_K302003 BBa_K302005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K302003 BBa_K302005 | 1,106 107,460 | BBa_K302003 BBa_K302005 |