BBa_K314973

BBa_K314973 Version 1

Component

This part has been discontinued.

Please consider using BBa_K314017 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K314973
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Wu, Christopher Eiben
Date created: 2010-10-17 11:00:00
Date modified: 2015-05-08 01:11:55

cappy



    • Details

    Types
    DnaRegion

    Roles
    Measurement

    engineered_region

    Sequences BBa_K314973_sequence (Version 1)

    Description

    A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.

    Notes

    Point mutation at residues 24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Asparagine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel

    Source

    In-House Mutant

    Sequence Annotation Location Component / Role(s)
    starty
    		54,76
    		feature/start start_codon

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    isReplacedBy
    BBa_K314017/1
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K314973/1
     

    Attachments

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