BBa_K315002

BBa_K315002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K315002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nitya Rao
Date created: 2010-06-14 11:00:00
Date modified: 2015-05-08 01:11:55

variant reverse lox site with 2 base changes in the spacer region (lox5171)



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K315002_sequence (Version 1)

Description

The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If one site is forward and the other reverse, the coding sequence will be inverted.

Notes

In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.

Source

We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735).

Sequence Annotation Location Component / Role(s)
Arm (Inverted Repeat)
Spacer Region
Arm (Inverted Repeat)
1,13
14,21
22,34
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K315002/1