BBa_K315025

BBa_K315025 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K315025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brianna Pearson
Date created: 2010-07-14 11:00:00
Date modified: 2016-02-10 02:44:56

pTet-loxBri(F)-RBS-RFP-loxN



    • Details

    Types
    DnaRegion

    Roles
    sequence_feature

    DNA

    Sequences BBa_K315025_sequence (Version 1)

    Description

    This device shows the activity of Cre on two variant lox sites, one with three point mutations (loxBri) in the inverted arm region and one with two (loxN). If these arms are compatible, which they shouldn't be, then RFP will be excised and cells will no longer glow red in the presence of Cre.

    Notes

    pTet is a moderate promoter and floxed RFP is an immediate indicator of excision.

    Source

    GCAT-alog of Davidson College.

    Sequence Annotation Location Component / Role(s)
    BBa_R0040
    TetR 1
    -35
    TetR 2
    -10
    		1,54
    1,19
    20,25
    26,44
    43,48
    		feature/BioBrick engineered_region
    non_covalent_binding_site feature/binding
    feature/promoter promoter
    non_covalent_binding_site feature/binding
    feature/promoter promoter

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K315025/1
     

    Attachments

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