BBa_K316017

BBa_K316017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:11:56

LacI operator controlled TEV protease

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K316017_sequence (Version 1)

Description

TEV protease S219P autocatalysis resistant variant BBa_K316012. This part had been reversed for the 3' strand in order to reduce any read-through that may be caused by upstream elements. Expression of TEV protease is under control of enhanced hyperspank promoter BBa_K316000.

Notes

TEV protease is naturally found in Tobacco Etch Virus genome. This part contains the sequence codon optimized for expression in B.subtilis using mwg ??? eurofins http://www.eurofinsdna.com/home.html proprietary software.

Source

The part was produced by nucleotide synthesis by mwg ??? eurofins. The synthetic RBS was designed to work with BBa_K316000.

Components

Access	Instance	Definition
public	BBa_K316012	TEV
public	BBa_K316000	sRBS Pehs

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K316012	1,717	TEV
BBa_K316000	726,874	sRBS Pehs

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316017/1

Member of these Collections

• iGEM Parts Registry