BBa_K317000

BBa_K317000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K317000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yoshiyuki MURAKAMI
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:11:57

SRII (NpSRII)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K317000_sequence (Version 1)

Description

Halobacteria exhibit phototaxis responses to changes in light intensity and color using the seven-transmembrane retinylidene photoreceptors sensory rhodopsins I and II (SRI and SRII).Light-activated SRI and SRII transmit signals to their cognate transducers, HtrI and HtrII, respectively. The Htr proteins contain two transmembrane helices and cytoplasmic methyl-accepting and His-kinase-activating domains homologous to those of chemotaxis transducers of eubacteria,such as the Escherichia coli and Salmonella enterica serovar
Typhimurium Tsr and Tar, chemotaxis transducers for serine and aspartate, respectively. Sensory rhodopsin
II from Natronobacterium pharaonis (NpSRII) is very similar in spectroscopic and functional properties to the repellent receptor SRII in Halobacterium salinarum, and it has been found to be more stable in response to variation in external conditions such as pH and ionic strength. The NpSRII protein mediates
a repellent response to blue-green light (maximum λ,497 nm) when it is coexpressed with its transducer, NpHtrII, in H. salinarum. Also, when expressed in E. coli, NpSRII is capable of binding all-trans retinal to form a blue-green-lightabsorbing pigment.

Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication

Source

Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T))

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K317000/1