Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Rostain
Date created: 2010-10-07 11:00:00
Date modified: 2015-05-08 01:11:59
Lac promoter + wild type firefly luciferase (yellow-green colour)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K322238_sequence (Version 1) |
Description
This part is made of the lac promoter followed by wild type firefly luciferase. This part was submitted because BBa_I712019 contains 6 bases upstream of the start codon, which reduce protein expression by increasing distance between the ribosome binding site and the start codon. Thus, bacteria expressing this part should be brighter.Notes
Note that there is a LacZ fragment between the Lac promoter and the firefly luciferase translational unit.Source
Source was BBa_I712019, which itself comes from Promega PGL3 vector.| Access | Instance | Definition |
| public public public | BBa_J33207 BBa_J15001 BBa_K322237 | BBa_J33207 BBa_J15001 BBa_K322237 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_J15001 BBa_K322237 | 1,600 609,618 625,2277 | BBa_J33207 BBa_J15001 BBa_K322237 |