BBa_K322999

BBa_K322999 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K322999

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Marta Bereska

Date created: 2010-10-07 11:00:00

Date modified: 2015-05-08 01:12:00

Blue light sensor (lovTAP) + Reporter system

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K322999_sequence (Version 1)

Description

lovTAP is a blue light sensitive protein submitted by EPF Lausanne in 2009. This has both lovTAP and the reporter system, so there is no need to have two different plasmids in a cell to detect blue light.

Notes

This blue light sensor is a fusion of a blue light sensitive protein with TrpR. Using a TrpR deletion strain might improve light sensitivity.

Source

Strickland, D., Moffat, K. & Sosnick, T. R. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences 105, 10709-10714.

Components

Access	Instance	Definition
public	BBa_K360121	BBa_K360121
public	BBa_K191004	BBa_K191004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K360121	1,844	BBa_K360121
BBa_K191004	853,1806	BBa_K191004

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322999/1

Member of these Collections

• iGEM Parts Registry