BBa_K324001

BBa_K324001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K324001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julius Ho
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02

AraC regulatory protein in RFC25 format



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K324001_sequence (Version 1)

Description

This part consists of the AraC regulatory protein optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. The LVA rapid degradation tag from our source DNA, BBa_C0080, was also removed from the C-terminus. All modifications were made to BBa_C0080 by means of PCR with specially designed primer set.

Notes

The LVA tag was removed because this part's intended purpose, harvesting from cells and purification, required the protein to have a long lifespan. 6-his tag was added to the C-terminus to avoid isolation of incompletely translated peptides during Ni-affinity purification.

Source

refer to http://partsregistry.org/Part:BBa_C0080

Sequence Annotation Location Component / Role(s)
AraC in RFC25
1,924
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K324001/1