Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:06
hsa-miR-122 Binding site pattern (4BS)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K337003_sequence (Version 1) |
Description
hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching:
1st: CAAACACCATTGTCACACTC-A
perfect: CAAACACCATTGTCACACTCCA
2nd: CAAACACCATTGTCA-ACTCCA
perfect: CAAACACCATTGTCACACTCCA
Notes
The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition.Source
http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421| Sequence Annotation | Location | Component / Role(s) |
| Spacer 1st half Spacer 1st half Spacer 1st half Spacer 2nd half Spacer 2nd half Spacer 1st half Spacer 2nd half Spacer 2nd half | 1,15 52,66 103,117 88,102 37,51 155,169 140,154 192,206 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |