BBa_K345669

BBa_K345669 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K345669

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: sebastian Ramov

Date created: 2010-11-05 12:00:00

Date modified: 2015-05-08 01:12:08

psb1c3 -> BsiWI

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K345669_sequence (Version 1)

Description

This is a new backbone modified from psb1c3 to facilitate ligation independent cloning. This new backbone allows creation of parts and composites into any desired cloning standard.

Notes

The EcoRI and PstI restriction sites are inactivated by mutagenesis so as not to create two sets of these restriction sites. A BsiWI restriction site is created between the XbaI and SpeI restriction sites for ligation independent cloning.

Source

pSB1C3

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LIC site (BsiWI)	1,4	sequence_feature, feature/misc
LIC site (BsiWI)	2071,2072	sequence_feature, feature/misc
E.coli his operon terminator	22,114	feature/stem_loop, stem_loop
verification reverse(VR) primer binding site	157,176	feature/primer_binding, primer_binding_site
rep(pMB1)	261,875	ribosome_entry_site, feature/rbs
T0 terminator	1032,1147	stem_loop, feature/stem_loop
chloramphenicol resistance	1262,1831	sequence_feature, feature/misc
verification forward (VF2) primer binding site	1932,1952	primer_binding_site, feature/primer_binding
end of terminator	2009,2012	stem_loop, feature/stem_loop
stem loop	2022,2041	stem_loop, feature/stem_loop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K345669/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors