BBa_K353004

BBa_K353004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K353004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Virrueta
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:11

I0500 + sRNA2



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K353004_sequence (Version 1)

Description

This part is composed of an inducible arabinose promoter (I0500) and an sRNA sequence. This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of the sRNA2 transcript, and if no sRNA1 is present (see part ), this can then be translated into GFP.

Notes

We designed the sRNA2 tag. It was developed as to not interfere with E. coli's native genome and to be bound by RSID2 (see part BBa_k353003).

Source

This part is assembled from other parts found in the registry (except for the sRNA sequence).

Sequence Annotation Location Component / Role(s)
AraC
PC
Pbad
BBa_I0500
1,1079
1043,1114
1080,1210
1,1210
sequence_feature feature/misc
feature/promoter promoter
promoter feature/promoter
feature/BioBrick engineered_region
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K353004/1