Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gregory Meyer
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:12:11
Lac/Ara-1 IPTG Inducible Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K354000_sequence (Version 1) |
Description
Derived from the L-arabinose and Lactose operons, this synthetic promoter remains in the 'off' state due to the repression of the AraC protein. AraC is constitutively expressed in bacteria possessing the arabinose operon and as such is present in E. coli. When exogenous arabinose and/or IPTG is added, the AraC protein is freed from the promoter thus allowing RNA polymerase to bind and initiate transcription. Hence, addition of arabinose or IPTG will switch the promoter into the 'on' state.Notes
The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.Source
The promoter was synthesised via primer extension.| Sequence Annotation | Location | Component / Role(s) |
| -33 Hexamer AraC Binding Site Symmetrical Synthetic Operator -10 Hexamer Lac Operon operator Sequence | 38,43 2,37 44,57 58,66 72,102 | promoter feature/promoter non_covalent_binding_site feature/binding operator feature/operator promoter feature/promoter feature/operator operator |