BBa_K357003

BBa_K357003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K357003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elena Pasko
Date created: 2010-06-22 11:00:00
Date modified: 2015-05-08 01:12:11

3-oxoadipate enol-lactonase (also known as beta-ketoadipate enol-lactone hydrolase)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K357003_sequence (Version 1)

Description

In enzymology, a 3-oxoadipate enol-lactonase (EC 3.1.1.24) is an enzyme that catalyzes the chemical reaction

3-oxoadipate enol-lactone + H2O --> 3-oxoadipate

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is 4-carboxymethylbut-3-en-4-olide enol-lactonohydrolase. This enzyme participates in benzoate degradation via hydroxylation.

Notes

The sequence contains no restriction sites.

ORF information:

ORF_2 rf(4)(1,666)<<<, ORF_1 rf(1)(106,792)>>>, GC=64%, AT=36%

Source

SOURCE Pseudomonas putida KT2440 (genomic sequence)

AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,
Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
Duesterhoft,A., Tummler,B. and Fraser,C.
TITLE Complete genome sequence and comparative analysis of the
metabolically versatile Pseudomonas putida KT2440
JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002)

Sequence Annotation Location Component / Role(s)
Start Codon
Stop Codon
1,3
790,792
feature/start start_codon
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K357003/1