PB642(L-C)

BBa_K365010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K365010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: RENAULT Renaud

Date created: 2010-09-14 11:00:00

Date modified: 2015-05-08 01:12:15

Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K365010_sequence (Version 1)

Description

Phytochrome B was fused to a trimer of ClpX in order to tether substrate containing PIF to the protease upon light application, thus promoting its degradation.

Notes

We used Freiburg fusion standard (RFC-25) for in frame assembly.

Source

See subparts.

Components

Access	Instance	Definition
public	BBa_K365003	PhyB-642
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K365003	1,1926	PhyB-642
BBa_B0105	1927,1932	Scar 25
BBa_K365005	1933,1992	linker
BBa_B0105	1993,1998	Scar 25
BBa_K365004	1999,3090	ClpX
BBa_B0105	3091,3096	Scar 25
BBa_K365005	3097,3156	linker
BBa_B0105	3157,3162	Scar 25
BBa_K365004	3163,4254	ClpX
BBa_B0105	4255,4260	Scar 25
BBa_K365005	4261,4320	linker
BBa_B0105	4321,4326	Scar 25
BBa_K365004	4327,5418	ClpX

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K365010/1

Member of these Collections

• iGEM Parts Registry