BBa_K365013

BBa_K365013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K365013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: RENAULT Renaud
Date created: 2010-10-09 11:00:00
Date modified: 2015-05-08 01:12:15

ClpX trimer



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K365013_sequence (Version 1)

Description

We fused 3 ∆N-ClpX with 20 aa linker in order to increase the stability of ClpX pseudo-hexamer.

Notes

See subparts.

Source

∆N-ClpX was initially extracted from E. coli genome using colony PCR, then we deleted some restriction sites. We then used standard assembly protocols.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K365013/1