BBa_K368007

BBa_K368007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K368007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2010-10-23 11:00:00

Date modified: 2015-05-08 01:12:15

tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K368007_sequence (Version 1)

Description

eukaryots:
tet-on Promoter (prev TRE): inducible CMV promoter, inducible by doxycycline
TEV recogn. site: specific recognition site for TEV protease
N-degron: Protein degradation signal, if N-terminus is free
SF3b155: Splicing factor 3b, size: 155kDa; interacting with p14*

Notes

-> "Cut'N'Survive" system

Source

(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))

Components

Access	Instance	Definition
public	BBa_K368001	BBa_K368001
public	BBa_K368019	BBa_K368019

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K368001	1,444	BBa_K368001
BBa_K368019	451,1635	BBa_K368019

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K368007/1

Member of these Collections

• iGEM Parts Registry