BBa_K371001

BBa_K371001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K371001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yang Zhang

Date created: 2010-08-26 11:00:00

Date modified: 2015-05-08 01:12:15

pduAB(Propanediol utilization gene A+B)[RBS+pduA+RBS+pduB]

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K371001_sequence (Version 1)

Description

Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex.
PduAB consist of pdu A and pdu B wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon such as pdu JK, can be used to construct a empty proteinous microcompartment in bacteria.

Notes

The sequencing result show that the gene of pduAB has some silent mutations, which have no effect on the function of protein.

Source

pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	6,11	ribosome_entry_site, feature/rbs
pduA coding sequence	19,300	CDS, feature/cds
start codon	300,302	feature/start, start_codon
stop codon	298,300	stop_codon, feature/stop
pduB coding sequence	300,1112	CDS, feature/cds
RBS	285,292	feature/rbs, ribosome_entry_site
stop codon	1110,1112	stop_codon, feature/stop
start codon	19,21	start_codon, feature/start

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K371001/1

Member of these Collections

• iGEM Parts Registry