Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ruijun Zhu
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:16
pSB1C5 (1bp mutation of pSB1C3 to remove SacI)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K371051_sequence (Version 1) |
Description
This part is a plasmid bone, which has only a single site mutation compared with pSB1C3. The SacI site is mutated to allow this plasmid compatible to BBF EFC 53 standard.Notes
In order to make our working plasmid compatible to BBF RFC 53, we search the restriction enzymen site in pSB1C3 plasmid. And we found a SacI site in the pSB1C3. The site is between downstream of chloramphenicol and the T0 terminator. We think the mutation does not affect the regular function of pSB1C3 plasmid. So all of our following experiment of fusion protein was done in this new plasmid.Source
this part comes from the partsregistry BBa_pSB1C3| Sequence Annotation | Location | Component / Role(s) |
| suffix E. coli his operon terminator Stem loop Deletion of AC Verification reverse (VR) primer binding site rep (pMB1) ORI T0 terminator C->G silent muation Chloramphenicol resistance A->G silent mutation Extra C discovered in sequencing C->T mutation end of terminator Stem loop prefix | 1,21 22,93 30,64 34,35 157,176 261,875 276,276 1042,1147 1149,1149 1162,1821 1237,1237 1919,1919 1939,1939 2009,2012 2022,2041 2051,2072 | feature/dna sequence_feature feature/stem_loop stem_loop feature/stem_loop stem_loop feature/mutation sequence_alteration feature/primer_binding primer_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc point_mutation feature/s_mutation CDS feature/cds point_mutation feature/s_mutation feature/mutation sequence_alteration feature/mutation sequence_alteration stem_loop feature/stem_loop feature/stem_loop stem_loop sequence_feature feature/dna |