Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Constantinou, Emmelie Lidh, Nina Schiller, Johan Nordholm
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:17
Yeast copper chaperon protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K380008_sequence (Version 1) |
Description
Yeast copper chaperon protein (yCCS) is a help chaperon specific for copper/zinc superoxide dismutase located to the cytoplasm. yCCS has been shown to successfully mediate the delivery of copper ions to human superoxide dismutase 1 (SOD1) (Ahl ??????et al??????. 2003). yCCS generates fully metallized, active SOD1 proteins that in turn protects the cell from oxidative damage.For the human superoxide dismutase 1 (SOD1), see [[Part:BBa_K380007]].
Notes
The start and stop codon was removed and the gene was redesigned with BioBrick prefix and suffix according to assembly standard 25 using specifically designed primers and PCR. A restriction site for EcoRI at basepair number 228 was removed using site-directed mutagenesis PCRSource
Saccharomyces cerevisiae S288c Ccs1p (CCS1), mRNA (GeneBank: NM_001182535.1), 750 nucleotide gene. First identified and characterized by Culotta ??????et al??????. (1997).| Sequence Annotation | Location | Component / Role(s) |
| yeast copper chaperon for SOD s_d_mut | 1,744 257,257 | CDS feature/cds sequence_alteration feature/mutation |