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BBa_K389401

BBa_K389401 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K389401

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonas Aretz

Date created: 2010-10-10 11:00:00

Date modified: 2015-05-08 01:12:19

Sensitivity tuner + luciferase

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Details

Types	DnaRegion
Roles	engineered_region Reporter
Sequences	BBa_K389401_sequence (Version 1)

Description

This is a modified I746370 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.

Notes

arabinose promoter removed, GFP exchanged with luciferase compared to the original part I746370

Source

luciferase from pGL4.10luc2 (Promega)
the rest from the partsregistry

Components

Access	Instance	Definition
public public public public public public public	BBa_I746350 BBa_B0034 BBa_E1010 BBa_B0015 BBa_I746360 BBa_B0034 BBa_K389004	BBa_I746350 BBa_B0034 mRFP1 BBa_B0015 BBa_I746360 BBa_B0034 luc

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I746350 BBa_B0034 BBa_E1010 BBa_B0015 BBa_I746360 BBa_B0034 BBa_K389004	1,237 246,257 264,969 978,1106 1115,1205 1214,1225 1232,2887	BBa_I746350 BBa_B0034 mRFP1 BBa_B0015 BBa_I746360 BBa_B0034 luc

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K389401/1