BBa_K390003

BBa_K390003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K390003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cody Tramp

Date created: 2010-07-31 11:00:00

Date modified: 2015-05-08 01:12:19

pilA secretion tag for Synechocystis sp. PCC 6803

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K390003_sequence (Version 1)

Description

This is the secretion tag on the 5' end of the coding sequence of the pilA gene in Synechocystis sp. PCC 6803. The PilA protein is the major secreted protein in this species (Sergeyenko TV and Los D A. 2000. Identification of secreted proteins of the cyanobacterium Synechocystis sp. strain PCC 6803. FEMS Microbiology Letters 193: 213-216).

In Synechocystis sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).

Notes

none

Source

Based on genomic sequence. Part was created by annealing two oligionucleotides together.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	feature/start, start_codon
secretion tag - Synechocystis sp. PCC 6803	1,64	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K390003/1

Member of these Collections

• iGEM Parts Registry