Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jon Russel, Leah Itagaki
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:21
phsABC_generator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K393000_sequence (Version 1) |
Description
This part contains a ribosome binding site the phsABC gene from Salmonella enterica serovar Typhimurium and a transcription terminator. The phsABC gene is a thiosulfate reductase that causes production of hydrogen sulfide gas. Bang et al. (AEM 2000) expressed this gene in E. coli to precipitate Cadmium.Notes
PCR amplified off the pSB74 plasmid to make it RFC[10] compatible.Source
Cut out of the plasmid pSB74 (Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene (phsABC) from Salmonella enterica Serovar Typhimurium in Escherichia coli; Sang-Weon Bang, Douglas S. Clark, and Jay D. Keasling; Applied and Environmental Microbiology, September 2000, p. 3939-3944, Vol. 66, No. 9.| Access | Instance | Definition |
| public public public | BBa_K393002 BBa_K393001 BBa_B0015 | BBa_K393002 BBa_K393001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K393002 BBa_K393001 BBa_B0015 | 1,12 19,3639 3648,3776 | BBa_K393002 BBa_K393001 BBa_B0015 |