Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ling Li
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:22
T7 RNA Polymerase pulse generator
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K396007_sequence (Version 1) |
Description
This part generate T7 RNA Polymerase pulse when there is AHL induction.Notes
T7 RNA Polymerase is destabilized adding LVA tag based on BBa_K145001Source
lux box (BBa_R1062), CI434 operator site(BBa_R0052)| Sequence Annotation | Location | Component / Role(s) |
| LVA T7 RNA Polymerase His tag RBS weak Lux Box -35 cI434 OR2 -10 cI434 0R 1 R1062 Lux Box RBS weak C0052 LVA | 4,33 1,2703 2683,2700 2711,2716 2762,2786 2762,2767 2747,2761 2740,2745 2726,2740 2820,2856 2802,2822 2863,2868 2877,3542 3507,3539 | feature/tag tag CDS feature/protein feature/tag tag feature/rbs ribosome_entry_site non_covalent_binding_site feature/binding promoter feature/promoter operator feature/operator promoter feature/promoter feature/operator operator promoter feature/promoter feature/binding non_covalent_binding_site feature/rbs ribosome_entry_site feature/cds CDS feature/tag tag |