Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Seobeom Heo
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:12:23
mAAA (EcoRI site mutated Aryl Acyl-Amidase)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K400628_sequence (Version 1) |
Description
Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds.The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible.
When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color.
As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol.
Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter.
In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution.
Notes
This part was cloned into pSB1C3 vector and mAAA was mutated in two EcoRI sites.Source
It was identified from a genomic library of a soil bacterium, which was isolated using a selection media having p-acetaminophenol as a sole carbon source.| Sequence Annotation | Location | Component / Role(s) |
| mutated aryl acylamidase | 1,1494 | feature/protein CDS |