Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Polasko
Date created: 2010-09-27 11:00:00
Date modified: 2015-05-08 01:12:26
Strong Plant RBS (Kozak sequence) + EYFP from E0030
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K414004_sequence (Version 1) |
Description
In order for Enhanced Yellow Fluorescent Protein (EYFP)to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in EYFP.Notes
One consideration we made was the strength of the RBS we wanted upstream of the reporter. Evidence can be found in the literature that a spectrum of translational activity can be generated by tampering with the Kozak sequence in plants. Future teams may wish to investigate how they can tamper with their Kozak of designing a system that requires discrete but relative expression of genes initiated by the same promoters.Source
The source of EYFP was derived using modified primers containing the Kozak sequence designed to bind to the iGEM part BBa_E0430 (Conboy,2004)| Sequence Annotation | Location | Component / Role(s) |
| Kozak Kpn I Translational Start EYFP (E0030) Translational Stop 2x | 7,18 1,6 19,19 19,735 736,736 | feature/rbs ribosome_entry_site feature/misc sequence_feature feature/start start_codon CDS feature/cds stop_codon feature/stop |