View

BBa_K415005

BBa_K415005 Version 1

Component

This part has been discontinued.

Please consider using BBa_K415006 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K415005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Crystal McKenzie

Date created: 2010-06-17 11:00:00

Date modified: 2015-05-08 01:12:26

pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

Back

Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K415005_sequence (Version 1)

Description

This composite is composed of the parts BBa_K415000 and BBa_K082020. It can produce both luxR and mCherry. The production of luxR is repressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI.

Notes

The luxR generator is put second, in case the terminator of BBa_K415000 is leaky.

Source

Composite of BBa_K415000 and BBa_K082020

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0065 BBa_B0034 BBa_J06504 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_C0062 BBa_B0010 BBa_B0012	cI+luxR BBa_B0034 mCherry BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 luxr BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0065 BBa_B0034 BBa_J06504 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_C0062 BBa_B0010 BBa_B0012	1,97 106,117 124,837 846,925 934,974 983,1036 1045,1056 1063,1818 1852,1931 1940,1980	cI+luxR BBa_B0034 mCherry BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 luxr BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Discontinued
igem#status	Deleted
isReplacedBy	BBa_K415006/1
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K415005/1