| Types | DnaRegion
|
| Roles | Tag
tag
|
| Sequences | BBa_K416003_sequence (Version 1)
|
Description
This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS.
Notes
There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
Source
This part was sent to use from Dr. Sheldon Park of the University of Buffalo.
