Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-RBS+RFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510015_sequence (Version 1) |
Description
The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a RBS+RFP cassette (BBa_K093005) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism using the transposase machinery of the Tn7 transposon.Notes
The BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm-RBS+RFP by EcoRI and PstI clonning.Source
Cloning of BBa_K093005 in the BCS of BBa_K510012.| Access | Instance | Definition |
| public public | BBa_K510012 BBa_K093005 | BBa_K510012 RBS-RFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_K093005 | 1,4549 4558,5281 | BBa_K510012 RBS-RFP |