Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
Delta-mCherry-4xFF4 Juxtacrine Signaling Ligand MammoBlock
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K511303_sequence (Version 1) |
Description
This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein. Additionally, the protein is succeeded by four copies of the FF4 intronic miRNA binding sequence, allowing for miRNA-mediated repression of this part's translation.Figures 1 and 2 below show fluorescent output from the Delta-mCherry protein, while Figures 3 and 4 below show transactivation of the Notch-Gal4-ESN receptor by the Delta-mCherry protein.
Notes
Due to the high secondary structure of FF4 miRNA, this part was assembled with standard restriction cloning methods, specifically using enzymes AscI and BamHI.Source
This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria; the sequence for the four FF4 micro RNA (miRNA) tags was synthetically produced.| Sequence Annotation | Location | Component / Role(s) |
| Delta-3 Juxtacrine Signaling Ligand mCherry Fluorescent Protein Stop Codon Four FF4 Binding Sites | 1,2142 2143,2850 2851,2853 2862,2949 | CDS feature/protein CDS feature/protein feature/stop stop_codon feature/tag tag |