Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K511818_sequence (Version 1) |
Description
This MammoBlock composite device produces the Notch-Gal4-ESN juxtacrine signaling receptor when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Notch-Gal4-ESN at a low, basal (OFF) level. This device is applicable to cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.Source
Consult constituent components.| Access | Instance | Definition |
| public public public | BBa_K415506 BBa_J85600 BBa_K511300 | BBa_K415506 BBa_J85600 BBa_K511300 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K415506 BBa_J85600 BBa_K511300 | 1,330 331,352 353,6427 | BBa_K415506 BBa_J85600 BBa_K511300 |