BBa_K511819

BBa_K511819 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K511819
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31

Inducible Yellow Fluorescent Protein Generator (UAS-Gal4-Citrine) MammoBlock Device



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K511819_sequence (Version 1)

Description

This MammoBlock composite device produces the yellow/green fluorescent protein Citrine when induced with Gal4 transactivator variants and otherwise produces Citrine at a low, basal (OFF) level.

Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. As an additional note for this part, we made use of an expression vector generated by the Elowitz lab in our initial experiments, and did not convert the Citrine sequence into an entry vector format.

Source

Consult constituent components.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K511819/1