BBa_K514000

BBa_K514000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K514000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Arcanum

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:12:32

C6-3OXO-HSL -> β
-galactosidase activity

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K514000_sequence (Version 1)

Description

This BioBrick is a composition of F2620 with I732019. It was conceived to characterize the construction F2620: check which concentration of its specific quorum sensing signal (C6-3-oxo-HSL) it's able to detect, and how long does it take the bacterium to produce an answer (in this case, the expression of lacZ).

Notes

It has been very difficult to join F2620 with I142019 by the 3A method. In fact, it has been difficult for all BioBricks. Maybe it's a good method for a machine, because you don't have to purify from electrophoresis gel, but DNA concentrations suffer a great decay in the process. Use the standard method.

Source

Parts distribution of 2011.

Components

Access	Instance	Definition
public	BBa_F2620	BBa_F2620
public	BBa_I732019	BBa_I732019

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_F2620	1,1061	BBa_F2620
BBa_I732019	1070,4299	BBa_I732019

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K514000/1

Member of these Collections

• iGEM Parts Registry