Bcr

BBa_K524100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K524100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shek Chin Hong

Date created: 2011-09-26 11:00:00

Date modified: 2015-05-08 01:12:34

Bcr - multidrug efflux system

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K524100_sequence (Version 1)

Description

Bcr is a multidrug efflux pump that belongs to the major facilitator superfamily (MFS), native to Escherichia coli. When overexpressed in E. coli, it is known to confer varying degree of resistance to several antibiotics, including bicyclomycin (selection-capable); tetracycline (8-fold increase in MIC*); kanamycin (4-fold increase in MIC*); fosfomycin (4-fold increase in MIC*); and acriflavine (2-fold increase in MIC*).

*: Compared with wild type

Notes

'GTG' start codon has been mutated to 'ATG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.

Source

Amplified from genomic DNA of Escherichia coli K-12 Strain RR1 via PCR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
bcr	1,1188	feature/cds, CDS
start	1,3	feature/start, start_codon
stop	1189,1194	stop_codon, feature/stop
B0010	1276,1355	feature/stem_loop, stem_loop
B0012	1364,1404	feature/stem_loop, stem_loop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K524100/1

Member of these Collections

• iGEM Parts Registry