Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Wittchen
Date created: 2011-10-24 11:00:00
Date modified: 2015-05-08 01:12:34
Fusion Protein of S-Layer SbpA and LigA
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K525006_sequence (Version 1) |
Description
Fusion Protein of S-Layer SbpA and LigANotes
Assembled via Freiburg Standard Assembly (RFC 25)Source
Assembled fusion protein. SbpA was synthesized, LigA is the natural ligase A of E. coli| Sequence Annotation | Location | Component / Role(s) |
| TAA K525401 T7 Promotor lac operator B0034 SbpA domain ATG Freiburg Scar K525720 LigA domain NgoMIV AgeI RBS | 5214,5217 73,3193 1,20 20,44 53,64 73,3192 73,75 3193,3198 3194,5217 3199,5208 76,82 5209,5215 56,59 | feature/stop stop_codon feature/BioBrick engineered_region promoter feature/promoter feature/operator operator engineered_region feature/BioBrick CDS feature/protein feature/start start_codon feature/misc sequence_feature feature/BioBrick engineered_region CDS feature/protein non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs |