Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sung Kuk Lee
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:35
Pompc promoter coding fimE
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K526000_sequence (Version 1) |
Description
Pompc is a promoter sequence that is regulated by the famous hybrid light receptor Cph8.fimE is a invertase,by function,that is capable of inverting any DNA sequence that is flanked by fimE recognition site.
When fimE recognition sequences are flanked to a promoter element we can ensure that there is no leaky expression as the promoter is in a reverse orientation thus, fim inversion promoters ensures that there is no leaky expression.
The hybrid light receeptor Cph8 mediated regulation of gene expression suffers from a high leaky expression. Hence, we made an attempt to reduce the leaky level of the light mediated gene expression systems by using a combination of light receptor and fimE processor.
In this biobrick part, we have made an attempt to regulate the expression of fimE based on light so that we could precisely turn on a previously uninduced gene . This system would ensure more tight control of target gene expression. This combination would be potentially useful for toxic gene expression.
Notes
There is no special design consideration. We can amplify the Pompc and fimE from E. coli genomic DNA and assembly them usingon's Assembly.Source
Both fimE and Pompc was amplified from E. coli genomic DNA.Ham, T.S., Lee, S.K., Keasling, J.D. and Arkin, A.P. A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Biotechnology and Bioengineering 94 (2006), pp. 1-4.