Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eva Meier Carlsen
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:35
Biosynthesis/Oximes/CYP79A2/Plug 'n' Play
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K527001_sequence (Version 1) |
Description
This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
Notes
Mutated at a Pst1 restiction site (nucleotide 342)No prefix and suffix.
User assembly ends.
Source
Institute of Plant Biology and BiotechnologyFaculty of LIFE Science
University of Copenhagen
Denmark
http://2011.igem.org/Team:DTU-Denmark-2