Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-10-22 11:00:00
Date modified: 2015-06-15 01:18:23
safety version of pSB1C3 plasmid
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K533012_sequence (Version 1) |
Description
The plasmid has a suicide gene and a mutation-controller sequence. It can hence eliminate devious mutations and help to restrain biobricks to only our engineered organisms.Notes
Do not use commonly used commercial competent cells when using this plasmid. Special strains are required.Source
derived from pSB1C3| Sequence Annotation | Location | Component / Role(s) |
| ccdB new EcoRI site mutated EcoRI -10 -35 stem_loop pMB1 Chloramphenicol resistance E. coli his operon terminator mutated I-SceI site VR VF2 | 2061,2366 2590,2595 2049,2054 2527,2532 2558,2563 2022,2041 261,874 1109,1819 22,93 2571,2588 157,176 1933,1952 | CDS feature/cds sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter feature/promoter promoter feature/stem_loop stem_loop sequence_feature feature/misc CDS feature/cds stem_loop feature/stem_loop sequence_feature feature/misc feature/primer_binding primer_binding_site primer_binding_site feature/primer_binding |