Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boxuan Zeng
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
promoter of ILV2
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K563002_sequence (Version 1) |
Description
Promoter of ILV2, under control of transcription factor Gcn4. In our project, when When TORC1 is inhibited by FAP, dephosphorylated Gcn4 would bind with pHIS4 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gcn4 and mTor.Notes
Design NcoI and XhoI at two ends of the promoterSource
Amplified from the genome of S.cerevisiae by PCR| Sequence Annotation | Location | Component / Role(s) |
| promoter of ILV2 | 1,509 | promoter feature/promoter |