BBa_K563013

BBa_K563013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K563013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Boxuan Zeng

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:42

Tor2 with mutation I1957T and E2360K

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K563013_sequence (Version 1)

Description

It has the mutation of I1957T and E2360K. It has high kinase activity, resist to rapamycin, amino starvation and multi-inhibitor. It will phosphorylate downstream transcription factors,and inhibit their nuclear location. In our project when inhibitors exist, TORC1 is inactive, mutated Tor will help it grow in the environment.

Notes

Same to BBa_K563012

Source

We mutate BBa_K563010 by circular PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
I1957T	6451,6453	feature/mutation, sequence_alteration
E2360K	7660,7662	sequence_alteration, feature/mutation

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K563013/1

Member of these Collections

• iGEM Parts Registry